More than 1,000 stewards, volunteers, marshals and police officers will be on hand at any one time to manage mourners as they make their way through London.
This includes 779 professional stewards for each 8.5 hour shift, as well as 100 civil servant volunteer marshals, 40 scouts and 30 members of the first aid nursing yeomanry.
The welfare of those waiting in line will be attended to by 10 members of the Red Cross per shift, as well as 30 multi-faith pastors co-ordinated by Lambeth Palace.
There will also be six Samaritans on hand and Salvation Army volunteers providing tea and biscuits at Waterloo as people walk to the queue.
A total of 1,500 military personnel will be stationed around London to bolster crowd control efforts, and 600 St Johns ambulance volunteers in both the capital and Windsor.
Mourners will have access to 500 portaloos along the route of the queue and eight St Johns Ambulance first aid stations.
A DCMS spokesman said: “We want to help as many people as possible pay their respects to Her Majesty the Queen and are keeping thousands of mourners updated on the lying in state queue location through innovative means, including a specially designed live online tracker.
“There was an issue with the locations being shared using what3words because of a minor error. Within a few minutes of being notified, we fixed the glitch and the system is now functioning correctly.”